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Gene
Expression Analysis has proven very
effective tool for Target Discovery
Prioritization and Validation, Lead Compound
Selection and Optimization, Compound
Toxicological Profiling etc. To effectively
select and prioritize lead compound with
improved odds of utility, one must
characterize compound for both their
potential capability of adversely affecting
a desired target / non target tissues. Early
effect of unwanted side effects of
pharmacologically active substances is vital
to establishing rapid screens avoiding
costly failures in the clinical arena. e.g.
liver damage is still the leading reason for
withdrawing drugs from the market and for
abandoning lead candidate during
development. The present methods based on
usage of lab animals involve a full
assessment of all lead compounds using
current toxicological method. On the other
hand, high density Microarray analysis is
impractical because of its high cost and
time required for test in toxicological
method.
To
address this deficit, JRF has started Gene
Expression Analysis service using Real Time
PCR technology with the array of set of gene
markers for variety of toxicological end
points. JRF offer full range of service
which includes dosing of drug compound to
invivo in animal or invitro in cultured
cells, designing array of primers for set of
genes according to sponsor requirements,
Total RNA extraction from animal tissue or
cultured cells, cDNA synthesis of RNA,
RT-PCR / qRT-PCR run and statistical
analysis of Gene Expression data. We can
also offer service for deciphering disease
pathway for your compounds.
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